What Is Phage Isolation?

Are phages good?

HIV, Hepatitis C, and Ebola have given viruses a bad name, but microscopic phages are the good guys of the virology world.

Each phage specializes in overtaking certain strains of bacteria—for example, staph, strep, and E.

coli—which they attack and use as a host to multiply..

What is a phage approach?

Phage therapy is the use of bacteriophages to treat bacterial infections. This could be used as an alternative to antibiotics when bacteria develop resistance. Superbugs that are immune to multiple types of drugs are becoming a concern with the more frequent use of antibiotics.

Do phages infect humans?

Even though the bacteriophages do not infect human cells directly, they can mediate virulence gene transfer from pathogenic to non-pathogenic bacterial strains.

How do bacteriophages infect humans?

When the phage infects a new bacterium, it introduces the original host bacterium’s DNA into the new bacterium. In this way, phages can introduce a gene that is harmful to humans (e.g., an antibiotic resistance gene or a toxin) from one bacterium to another.

Is a temperate phage?

Temperate phages are bacteriophages that can choose between the lytic and the lysogenic pathways of development. The lytic pathway is similar to that of virulent phages. … When lysogeny is chosen, the phage integrates its genetic material with the host cell.

Are phages alive?

Bacteriophages, or “phages” for short, are viruses that specifically infect bacteria. Phages and other viruses are not considered living organisms because they can’t carry out biological processes without the help and cellular machinery of another organism.

How are phages isolated?

Phages are purified by removing, picking off, a well isolated plaque using either a Pasteur pipette or more crudely, but just as effectively, a wire loop. … Sodium azide can also be used for some phages. A second cycle of purification is required to ensure a single phage-strain population.

How do you isolate bacteriophage from soil?

With the enrichment method, all soil bacteria are removed from the soil extracted phage samples by filtration through a 0.22 µM filter. The sterile filtered lysate containing phage particles, but not soil bacteria, are diluted in 2x LB broth with the desired bacteria used as the phage propagation host.

How do you count phages?

The number of phage particles contained in the original stock phage culture is determined by counting the number of plaques formed on the seeded agar plate and multiplying this by the dilution factor. For a valid phage count, the number of plaques per plate should not exceed 300 nor be less than 30.